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Product Name Anti-ATEXPA10/AF594 Chinese Name AF594标记的Botany延长蛋白(拟南芥) Alias ARABIDOPSIS THALIANA EXPANSIN A 10; Alpha-expansin 10; At-EXP10; AtEx10; Ath-ExpAlpha-1.1. Product Type Botany抗体 Research Area Botany Immunogen Species Rabbit Clonality Polyclonal React Species Applications ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 28kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ATEXPA10 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
structural constituent of cell wall; INVOLVED IN: syncytium formation, plant-type cell wall modification during multidimensional cell growth,unidimensional cell growth, plant-type cell wall loosening; LOCATED IN: plant-type cell wall; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Barwin-related endoglucanase.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
延长蛋白 extensin是存在于Botany细胞壁中的羟脯氨酸(Hyp)占有高比例的glycoprotein。细胞壁中含达2—10%,Hyp占构成蛋白质的氨基酸的20%以上,在其他蛋白质中则丝氨酸(Ser)的含量高。由于可以控制Botany细胞的伸展(ex-tersion),故称为伸展蛋白。在生长旺盛的幼龄期的细胞中含量少,生长速度迟缓时含量增加,因而认为具有对细胞壁不易伸展的作用。其蛋白质部分具有-ser-Hyp-Hyp-Hyp-Hyp的氨基酸序列,Hyp残基许多有3—4个阿拉伯糖所构成的寡糖;而Ser残基许多具有1—2个半乳糖。脯氨酸羟化是在肽合成后进行,催化此过程的肽基脯氨酸羟化酶需要二价铁离子,可被铁离子络合剂的a′-dipyridyl 所抑制。肽的脯氨酸羟化后,细胞器的糖链结合,被分泌到细胞外,纳入细胞壁中。
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