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Product Name Anti-POU3F3/AF350 Chinese Name AF350标记的大脑蛋白1抗体 Alias Brain 1; Brain1; Brain specific homeobox/POU domain protein 1; Brain-1; Brain-specific homeobox/POU domain protein 1; Brain1; BRN 1; Brn-1; BRN1; class 3; Oct-8; Octamer-binding protein 8; Octamer-binding transcription factor 8; OTF 8; OTF-8; OTF8; PO3 F3; PO3F 3; PO3F3; PO3F3_HUMAN; POU class 3 homeobox 3; POU domain; POU domain, class 3, transcription factor 3; POU3 F3; POU3F 3; Pou3f3; RHS 1; Rhs 2; RHS1; Rhs2; Skin 1; Skin1; transcription factor 3. Research Area Cell biology Developmental biology Neurobiology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 50kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Brain1/POU3F3 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU domain proteins. Expressed during the development of the forebrain and coexpressed in most layer II-V cortical neurons, Brn-1 and Brn-2 appear to critically control the initiation of radial migration of cortical neurons. Brn-2 is thought to be involved in smooth muscle cell development and differentiation. Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. Brn-3a has two functional transactivating domains, one at the amino terminus and one at the carboxy terminus. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor.
Function:
Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3').
Subcellular Location:
Nucleus.
Tissue Specificity:
Brain.
Similarity:
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Database links:Entrez Gene: 5455 Human
Entrez Gene: 18993 Mouse
Omim: 602480 Human
SwissProt: P20264 Human
SwissProt: P31361 Mouse
Unigene: 673855 Human
Unigene: 440553 Mouse
Unigene: 483029 Mouse
Unigene: 11354 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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