Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

       Kit features:

1, Specific detection of Waddlia chondrophila, no cross-reaction with other biological genomes.

2, High sensitivity, the lowest detection limit is about 3 IFU/response.

3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, can be used Check the effectiveness of the kit.

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.

 

Chlamydia avium was first isolated from pigeons and parrots in Germany and Italy until the 20th centuryIt was only identified as a new species in 14 years and is currently only found in pigeons and parrots. Infection in poultry is usually asymptomatic and sometimes causes weight loss, fluffy feathers, anorexia, difficulty breathing, hepatosplenomegaly, and even death. According to a study on wild pigeon feces, the detection rate of Chlamydia avium is about 20-36%, which is higher than the 2.4-7.5% level of Chlamydia psittaci . The extent of the hazard to humans is unknown.

 

The detection of avian chlamydia generally uses the PCR method. PCR is a method of enzymatically synthesizing specific DNA fragments in vitro. It is more sensitive than other methods and has strong specificity. It can determine the type of bacterial infection. The detection speed is fast and can be completed in only two or three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

 

This kit designs primers and probes based on the gene sequence of enolase. It can specifically identify Chlamydia avium, and has been verified by BLAST to have no cross-reactivity with other biological genomes. This kit detected 6 other chlamydia, 19 bacteria and 3 fungi, and no non-specific signals were found. 125 pigeon cloacal swab samples that were positive for chlamydia were tested and 10 positive results were obtained. It can be seen that this kit is suitable for the detection and identification of Chlamydia avianum.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1，   特异性检测鸟衣原体，与其他生物基因组无交叉反应。

2，   灵敏度高，最低检测极限约为3 IFU/反应。

3，   DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4，   带有阳性对照样品，可用于检验试剂盒有效性。

5，   带有UDG酶和dUTP，可降低残留DNA的污染。

 

鸟衣原体（Chlamydia avium）最初是在德国和意大利从鸽子和鹦鹉中分离出来，直到2014年才确定为新物种，目前仅在鸽子和鹦鹉中发现有分布。家禽感染后通常没有症状，有时会引起体重下降、羽毛蓬松、厌食、呼吸困难、肝脾肿大，甚至死亡。根据一项对野生鸽粪便的研究，鸟衣原体的检测感染率在大约20-36%的水平，高于鹦鹉热衣原体（Chlamydia psittaci）2.4-7.5%的水平，目前尚不清楚对人类的危害程度。

对鸟衣原体的检测一般采用PCR法。PCR是一种体外酶促合成特异性DNA 片段的方法,灵敏度高于其他方法，特异性强，可以确定感染的细菌种类，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对烯醇化酶的基因序列设计引物和探针，可以特异性识别鸟衣原体，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了6种其他衣原体、19种细菌和3种真菌，未发现非特异性信号；对衣原体阳性的125个鸽泄殖腔拭子样本进行检测，获得10个阳性。可见本试剂盒适用于鸟衣原体的检测和鉴定。

 

 

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