Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Chlamydia abortus, also known as Chlamydophila abortus/Chlamydophila abortus or ovine or bovine abortion Chlamydia is an obligate intracellular parasitic prokaryotic organism that is Gram-negative. It was first discovered in 1950. It was once classified as a subtype of Chlamydia psittaci and was discovered in 1999. officially established as an independent species. Chlamydia abortus mainly accumulates in the placenta, causing miscarriage, premature birth or stillbirth. It is the most common abortion factor in goats and sheep. It can also cause long-term subclinical infection in non-pregnant sheep. In sheep with no history of exposure, Chlamydia abortus can cause 25-60% of ewes to miscarry. In sheep in epidemic areas, the abortion rate can be reduced to 1-15%, and mainly occurs in exotic sheep and primiparous sheep. . Chlamydia abortus can parasitize a variety of mammals. In addition to ruminants, there are also pigs, horses, deer, rabbits, guinea pigs, mice, foxes, minks, raccoon dogs, etc., and also Can infect non-mammals . Chlamydia abortus is endemic and has been found in many countries around the world, often causing significant economic losses. Infected animals may also infect humans, causing miscarriages and respiratory disorders, thus posing a threat to public health.

 

For the detection of Chlamydia abortus, conventional methods include: in vitro isolation and culture, immunohistochemistry As well as serological methods, etc., the disadvantages are that they are time-consuming and have insufficient sensitivity and specificity. PCR is an in vitro enzymatic method for synthesizing specific DNA fragments. It has high sensitivity, strong specificity, and fast detection speed. It only takes two or three hours to complete, so it can effectively replace the above methods for detection.

 

This kit designs primers for the 16S-23S rRNA gene interval sequence, which can be specific Sexual identification of Chlamydia abortus, verified by BLAST, only cross-reacts with the Chlamydia buteonis genome. Chlamydia buteonis is a chlamydia isolated from the North American red-shouldered buzzard in the past two years., the genetic relationship is between Chlamydia abortus and Chlamydia psittaci. Considering the differences in hosts, it generally does not affect the detection of Chlamydia abortus in mammals. This kit detected 9 different types of chlamydia, 20 types of bacteria and 4 types of viruses that may cause similar symptoms, and found no non-specific signals; in 3 chlamydia-positive sheep samples, 1 was found to be positive for chlamydia abortus. This kit can be used for the detection and identification of Chlamydia abortus.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

流产衣原体（Chlamydia abortus），也称为流产嗜衣原体/流产亲衣原体（Chlamydophila abortus）或羊牛流产衣原体，是一种专性细胞内寄生的原核生物，革兰氏阴性，1950年首次发现，曾被归类为鹦鹉热衣原体（Chlamydia psittaci）的一个亚型，于1999年正式确立为独立物种。流产衣原体主要聚集在胎盘，引起流产、早产或死产，是山羊和绵羊中最常见的流产因素，在非孕期羊中也会造成长期的亚临床感染。在无暴露史的羊群中，流产衣原体可引起25-60%的母羊流产，在疫区羊群中，流产率则可降到1-15%，并且主要发生在外来羊和初产羊。流产衣原体可寄生于多种哺乳动物，除了反刍动物（如：山羊，绵羊，牛和鹿）以外，还有猪、马、鹿、兔、豚鼠、小鼠、狐、貂、貉等，并且还可以感染非哺乳动物（如鸟类）。流产衣原体有地方流行性，全球许多国家都有发现，常造成重大经济损失。受感染的动物还可能感染人类，造成流产和呼吸功能紊乱，因此也会对公共健康造成威胁。

流产衣原体的检测，常规方法有：体外分离培养、免疫组化以及血清学方法等，缺点在于耗时，灵敏度和特异性都有所不足。PCR是一种体外酶促合成特异性DNA 片段的方法,灵敏度高，特异性强，检测速度快，只需两三个小时即可完成，因此可以有效替代上述方法进行检测。

本试剂盒针对16S-23S rRNA基因区间序列设计引物，可以特异性识别流产衣原体，经BLAST验证，仅与Chlamydia buteonis基因组有交叉反应。Chlamydia buteonis是近两年从北美赤肩鵟分离出来的衣原体，亲缘关系处于流产衣原体和鹦鹉热衣原体之间，考虑到宿主的差异，一般不会影响到哺乳动物流产衣原体的检测。本试剂盒检测了9种不同的衣原体，和可能引起类似症状的20种细菌和4种病毒，未发现非特异性信号；在3个衣原体阳性绵羊样本中，发现1个流产衣原体阳性。本试剂盒可用于流产衣原体的检测和鉴定。

 

 

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