Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Chlamydia psittaci, also known as Chlamydia psittaciphila / Chlamydia psittaciphila, parrot Chlamydia is a strictly intracellular parasitic prokaryotic organism belonging to the family Chlamydiaceae . Chlamydia psittaci is the earliest chlamydia discovered. It has a wide range of hosts, including not only a variety of birds, including parrots, but also many mammals; unique subtypes have been formed in many hosts. Chlamydia psittaci is divided into many genotypes, including: A–F, M56 and WC. Each subtype has strict host specificity. The host of subtype A is Psittacidae birds, the host of subtype B is pigeon, and the host of subtype C is pigeon. The hosts of subtype D are ducks and geese, the hosts of subtype D are turkeys, the hosts of subtype E are pigeons, ducks and other birds, the hosts of subtype F are parrots, the hosts of subtype WC are cattle, and the hosts of subtype M56 are rodents. animal. There are also some subtypes that have been divided into independent species, such as: Chlamydia pecorum), Chlamydia abortus, guinea pig Chlamydia, Chlamydia felis, etc. Chlamydia psittaci can cause respiratory disease in birds and mammals through direct contact, guano, nasal secretions, and aerosols; it can pose a public safety threat because it can remain infectious in the environment for months. These include large-scale outbreaks in poultry farming and occasional psittacosis in humans. Humans are generally infected from domestic or wild birds, and human-to-human transmission has yet to be confirmed. Human infections often have no symptoms or flu-like symptoms, and sometimes develop into severe pneumonia, endocarditis or encephalitis. .

 

Chlamydia psittaci can be detected using culture and serological methods. Chlamydia psittaci is easy to grow in the culture of chicken embryo yolk sac, HeLa cells, and monkey kidney cells. However, the culture method is rarely used because it is time-consuming and laborious and requires high skills and equipment of the experimenter. some serum prescriptionsMethods, such as complement fixation test, indirect hemagglutination test technology, ELISA, and microimmunofluorescence method, are often used to detect Chlamydia psittaci. The disadvantage is that it may cross-react with other chlamydiae and has low sensitivity, making it unsuitable for early infection. The PCR method is an in vitro enzymatic synthesis method of specific DNA fragments. It is more suitable for detecting Chlamydia psittaci. It has high sensitivity, strong specificity, and fast detection speed, which can be completed in only two or three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

 

This kit designs primers for the 16S-23S rRNA gene interval sequence, which can be specific Sexually identifying Chlamydia psittaci, verified by BLAST, there is no cross-reactivity with other biological genomes. This kit was used to detect 9 different types of chlamydia, 20 types of bacteria and 4 types of viruses that may cause similar symptoms, and no non-specific signals were found; 327 bird samples were tested and 47 positive results were obtained. It can be seen that this kit is suitable for the detection and identification of Chlamydia psittaci.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

鹦鹉热衣原体（Chlamydia psittaci），或称鹦鹉热嗜衣原体/鹦鹉热亲衣原体（Chlamydophila psittaci）、鹦鹉衣原体，是一种严格细胞内寄生的原核生物，属于衣原体科（Chlamydiaceae）。鹦鹉热衣原体是最早发现的衣原体，宿主非常广泛，不仅有包括鹦鹉在内的多种鸟类，还有许多哺乳动物；在许多宿主中已形成独特的亚型。鹦鹉热衣原体分为很多基因型，包括：A–F, M56和WC，每种亚型都具有严格的宿主特异性，A亚型宿主为鹦鹉科鸟类，B亚型宿主为鸽，C亚型宿主为鸭和鹅，D亚型宿主为火鸡，E亚型宿主为鸽、鸭及其他鸟类，F亚型宿主为鹦鹉，WC亚型宿主为牛，M56亚型宿主则为啮齿类动物。还有一部分亚型已被划分为独立物种，如：兽类衣原体（Chlamydia pecorum）、流产衣原体（Chlamydia abortus）、豚鼠衣原体（Chlamydia caviae）、猫衣原体（Chlamydia felis）等。鹦鹉热衣原体的传播可通过直接接触、鸟粪、鼻分泌物和气溶胶，引起鸟类和哺乳动物的呼吸道疾病；由于可在环境中保持感染力达数月之久，因此可以造成公共安全威胁，包括在家禽养殖中的大规模爆发，以及在人类中偶尔引发的鹦鹉病（或称鸟疫、鹦鹉热）。人类一般是从家养或野生鸟类获得感染，人与人之间的传播尚待证实，人类感染后常没有症状或出现类似流感的症状，有时会发展为严重肺炎、心内膜炎或脑炎。

 鹦鹉热衣原体可使用培养法和血清学方法进行检测。鹦鹉热衣原体在鸡胚卵黄囊及HeLa细胞、猴肾细胞培养中易于生长，但由于耗时费力，对实验者技能和设备要求也比较高，因此培养法很少使用。一些血清方法，如：补体结合试验、间接血凝试验技术、ELISA、微量免疫荧光法，常用于检测鹦鹉热衣原体，其缺点在于可能与其他衣原体产生交叉反应，且灵敏度较低，不适用于早期感染。PCR法是一种体外酶促合成特异性DNA 片段的方法,更适合检测鹦鹉热衣原体，其灵敏度高，且特异性强，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对16S-23S rRNA基因区间序列设计引物，可以特异性识别鹦鹉热衣原体，经BLAST验证，与其他生物基因组没有交叉反应。使用本试剂盒检测了9种不同的衣原体，和可能引起类似症状的20种细菌和4种病毒，未发现非特异性信号；对327个鸟类样本进行检测，获得47个阳性。可见本试剂盒适用于鹦鹉热衣原体的检测和鉴定。

 

 

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