Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Chlamydiaceae belongs to the Chlamydia phylum and the Chlamydia order , first established in 1966. In 1999, based on the RNA gene sequences of only four members, they were divided into two species: Chlamydia and Chlamydophila . As more chlamydia species were discovered, the differences between the two species were no longer obvious, so in 2010 they were officially merged into one genus, still called Chlamydia . . Chlamydia was first discovered in 1935. Currently, about 15 species have been discovered, all of which are obligate intracellular parasites and can cause diseases in humans and animals. Chlamydia has been found in humans, mammals, birds and reptiles. Many chlamydiae can infect multiple hosts, while some are host-specific and infect only one type of host or type of host. The chlamydia that can infect humans are: Chlamydia trachomatis, Chlamydia pneumonia, Chlamydia psittaci, Chlamydia abortus, etc. Chlamydia mainly invades the mucous membranes of the respiratory tract, gastrointestinal tract or reproductive system. The site and severity of infection are related to the type of chlamydia and the host. The infection sometimes does not show symptoms, but in most animals it causes conjunctivitis and nasal mucositis, or it invades the reproductive system and causes miscarriage and infertility. Birds infected with C. psittaci often have a subclinical state, but under stress may develop clinical symptoms, manifesting as severe pneumonia and organ failure. Humans can become infected while raising birds, producing flu-like symptoms, often leading to misdiagnosis and serious consequences.

 

Chlamydia can be detected by culture and serological methods. Since chlamydia is an obligate intracellular parasite, it mustIt must be cultured in living tissue or cells, which is complex, time-consuming and has low sensitivity. Serological methods are also less sensitive and can only detect after infection has been present for some time. The PCR method has the advantages of high sensitivity, strong specificity, and fast detection speed, which can be completed in only two to three hours, which can effectively make up for the shortcomings of the above two methods.

 

This kit designs specific primers and The probe can specifically identify various Chlamydiae/Chlamydiaphila, and has been verified by BLAST to have no cross-reactivity with the genomes of other organisms other than Chlamydiaceae. This kit detects 9 types of chlamydia, 20 types of bacteria and 4 types of viruses that are prone to produce similar clinical symptoms. Only chlamydia produces specific signals and has no cross-reactivity with other bacteria and viruses. 833 bird tissue samples were tested in parallel using three methods. The fluorescent-labeled monoclonal antibody method obtained 54 positives, the modified Ziehl-Neelsen staining method obtained 40 positives, and this kit obtained 103 positives, including all the previous ones. The positive samples obtained by the two methods show that this kit has higher sensitivity.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

衣原体科（Chlamydiaceae）属于衣原体门（Chlamydiae phylum）衣原体目（Chlamydiales order），于1966年首次确立。1999年根据仅有的4个成员的RNA基因序列，将其分为两个种属：衣原体属（Chlamydia）和嗜衣原体属(Chlamydophila)。随着更多衣原体种类的发现，两个种属之间的区别不再明显，因此2010年正式将二者重新合并为一个种属，仍称为衣原体属（Chlamydia）。衣原体最早发现于1935年，现在已发现的有约15种，全部是专性细胞内寄生，在人和动物中可引起疾病。目前已在人、哺乳动物、鸟类和爬行类动物中发现衣原体。很多衣原体可感染多种宿主，有些则具有宿主特异性，只感染一种或一类宿主。可感染人类的衣原体有：沙眼衣原体（Chlamydia trachomatis），肺炎衣原体（Chlamydophila pneumoniae），鹦鹉热衣原体（Chlamydophila psittaci），流产衣原体（Chlamydophila abortus）等。衣原体主要侵袭呼吸道、胃肠道或生殖系统的粘膜，感染位点和严重程度与衣原体种类和宿主都有关系。感染后有时并不表现出症状，在多数动物中会引起结膜炎和鼻粘膜炎，或者侵袭生殖系统导致流产和不育。鸟类感染鹦鹉热衣原体后常处于亚临床状态，但在压力下可能出现临床症状，表现为重度肺炎和器官衰竭。人类可在养鸟过程中被感染，产生类似流感的症状，常造成误诊导致严重后果。

衣原体可以通过培养法和血清学方法进行检测。由于衣原体是专性细胞内寄生，因此必须在活组织或细胞内进行培养，操作复杂，耗时，灵敏度低。血清学方法同样灵敏度较低，且只能在已感染一段时间之后才能检测。PCR法具有灵敏度高、特异性强的优点，检测速度快，只需两三个小时即可完成，可以有效弥补上述两种方法的不足。

本试剂盒针对16S-23S rRNA基因区间序列设计特异性引物和探针，可以特异性识别各种衣原体/嗜衣原体，经BLAST验证，与衣原体科以外的其他生物基因组没有交叉反应。本试剂盒检测了9种衣原体，和易于产生相似临床症状的20种细菌及4种病毒，只有衣原体产生特异性信号，与其他细菌和病毒没有交叉反应。对833个鸟类组织样本用三种方法进行平行检测，荧光标记单克隆抗体法获得54个阳性，改良Ziehl-Neelsen染色法获得40个阳性，而本试剂盒获得103个阳性，完全包括了前两种方法获得的阳性样本，可见本试剂盒具有更高的灵敏度。

 

 

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