Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

Mycoplasma anatis, also known as duck mycoplasma, is an intracellular parasite and belongs to the class Mollicutes Mycoplasma is a Gram-negative bacterium. Mycoplasma anagi was first discovered in ducks and can also be isolated from healthy geese. After infection, it may cause severe respiratory diseases. The main clinical symptoms are: sinusitis, airsacculitis, peritonitis, decreased growth rate, and embryonic death, and may be related to cloaca and Penile inflammation is associated with the most common symptom being a decrease in egg hatching success, resulting in economic losses. Mycoplasma anagi can be transmitted vertically through eggs.


Mycoplasma duck can be detected through culture method. The disadvantage is that culture is more difficult and the appearance It has a typical omelette shape and is difficult to distinguish from other mycoplasmas, so biochemical identification is required. Serological methods are time-consuming and require purified in vitro culture colonies, and antiserum is not easy to obtain. Using the PCR method has the advantages of high sensitivity and short detection time, and results can be obtained in just a few hours


The Mycoplasma duck PCR detection kit selects the DNA polymerase gene for PCR identification, and the primers It has been verified by BLAST that it specifically targets Mycoplasma anatipilum and has no cross-reactivity with the genomes of other organisms. This kit was used to detect 9 Mycoplasma analyxin strains and 15 other avian Mycoplasma species, and only 9 Mycoplasma anatipilum strains produced specific bands; 28 waterfowl samples were tested and 4 positive results were obtained, which was consistent with the sequencing results. . It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma duck.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

鸭支原体（Mycoplasma anatis）又称鸭霉形体，营胞内寄生，属于柔膜菌纲（Mollicutes）支原体科（Mycoplasmataceae），是一种革兰氏阴性菌。鸭支原体最先发现于鸭，健康鹅也可分离出来，感染后可能引起严重的呼吸道疾病，主要临床症状为：鼻窦炎，气囊炎，腹膜炎，生长速度下降，以及胚胎死亡，并且可能与泄殖腔和阴茎炎症有关，其中最常见的症状是孵卵成功率下降，从而导致经济损失。鸭支原体可通过卵进行垂直传播。

鸭支原体可通过培养法进行检测，缺点是培养较为困难，外观为典型的煎蛋状，与其他支原体不易区分，需进行生化鉴定。血清学方法则耗时较长，需要纯化的体外培养菌落，抗血清也不易获得。使用PCR法则具有高灵敏度的优点，且检测时间短，仅需几个小时即可获得结果。

鸭支原体PCR检测试剂盒选取DNA聚合酶基因进行PCR鉴定，引物经BLAST验证为特异性靶向鸭支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了9个鸭支原体菌株以及15种其他鸟类支原体，仅有9个鸭支原体菌株产生特异性条带；对28个水禽类样本进行检测，获得4个阳性，同测序结果一致。可见本试剂盒具有物种特异性，可用于鸭支原体的鉴定和检测。

 

 

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