Determination of Significance：

Phospholipase C (EC 3.1.4.3) is a kind of lipid hydrolase that hydrolyzes glycerophosphate bond at C3 site of glycerophosphate. It is widely existed in the tissues and cells of microorganisms, animals and plants, and plays an important role in cell metabolism, cell transmission, growth and development.

Measurement Principle：

Phospholipase C catalyzes the hydrolysis of NPPC to produce p-nitrophenol with a characteristic absorption peak at 410 nm.

Self Provided：

Visible spectrophotometer/ microplate reader, water bath, ultra fast freezing centrifuge, adjustable pipette, balance, micro glass cuvette/96-well plate, mortar/homogenizer/cell ultrasonicator, distilled water, ice.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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