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Calcium Assay Kit (methylthymol Blue colorimetric method)
Calcium Assay Kit (methylthymol Blue colorimetric method)
钙试剂盒 (甲基百里酚蓝比色法)
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Calcium is a metal element, which is a silver-white crystal at room temperature. Animal bones, clam shells and egg shells all contain calcium carbonate. The detection of calcium content in living organisms is mainly achieved by detecting the concentration of calcium ions. At present, the commonly used dyes include o-cresolphthalide complex ketone (OCPC), azo arsenic Ⅲ, methyl thymol blue (MTB), etc.

In this kit, calcium ions in solution can combine with MTB under alkaline conditions to form a sea blue/blue complex. Magnesium ion chelator was added to remove magnesium ion background interference. The absorbance at 610nm was detected by spectrophotometer, and the total calcium content was calculated according to the formula.


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