Summary:

 

Purpose

Our Quick Step Rat ADAR2/ADARB1 ELISA Kit is to assay ADAR2/ADARB1 levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ADAR2/ADARB1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ADAR2/ADARB1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ADAR2/ADARB1 and HRP conjugated ADAR2/ADARB1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ADAR2/ADARB1. You can calculate the concentration of ADAR2/ADARB1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QSD1884Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0	0.044	0.0480
		0.052
S1	150	1.967	1.9630
		1.959
S2	100	1.328	1.3195
		1.311
S3	50	0.692	0.7030
		0.714
S4	25	0.392	0.3875
		0.383
S5	12.5	0.197	0.1900
		0.183

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