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Drug Names
Generic Name:Human Cytochrome P450 11A1 (CYP11A1) ELISA Kit
Purpose
Our Human Cytochrome P450 11A1 (CYP11A1) ELISA kit is to assay CYP11A1 levels
in serum, plasma, culture media or any biological fluid. Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided
in this kit has been pre-coated with an antibody specific to CYP11A1. Standards or samples
are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for CYP11A1 is added to
each Microelisa stripplate well and incubated. Free components are washed away. The TMB
substrate solution is added to each well. Only those wells that contain CYP11A1 and HRP
conjugatedCYP11A1 antibody will appear blue in color and then turn yellow after the
addition of the stop solution. The optical density (OD) is measured spectrophotometrically at
a wavelength of 450 nm. The OD value is proportional to the concentration of CYP11A1. You can calculate the concentration of CYP11A1 in the samples by comparing the OD of the
samples to the standard curve.
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