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Drug Names
Generic Name:Human Reovirus antibody IgM(Reo- IgM)ELISA Kit
Purpose
Our human Reovirus antibody IgM(Reo- IgM)ELISA kit is to assay Reo- IgM levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Reo- IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Reo is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Reo- IgM and HRP conjugated Reo antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Reo- IgM is determined by comparing with the CUTOFF value.
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