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Drug Names
Generic Name: Human Dipeptidyl Peptidase (DPP) ELISA Kit (Enzyme activity)
Purpose
Our Human Dipeptidyl Peptidase (DPP) ELISA kit (Enzyme activity) is to assay DPP Enzyme Active in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to DPP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for DPP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain DPP and HRP conjugated DPP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of DPP. You can calculate the concentration of DPP in the samples by comparing the OD of the samples to the standard curve.
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