Product instruction:

α-L-Arabinofuranosidase, α-L-AF) belongs to sugar The base hydrolyzate family can generate a L-Arabic sugar molecule from a polymer containing a propanogenic glycer with arabinose such as arabinopan, arabant glycans, and arabic semi-lactose. This enzyme has an important role in hemicellulose degradation and the maturity of fruits.

α-L-AF decomposition to nitrophenyl-α-L-araboside is a pair of nitrophenol, and the maximum absorption peak is at 400 nm at 400 nm, by determining 400 nm Changes in the absorbance value can be calculated to be alpha-L-AF activity.

The instrument and reagent required in the test

:

Variable spectrophotometer / enzyme label, a desktop low temperature centrifuge, Water bath / thermostatic incubator, trace glass cuvette / 96-well plate, adjustable pipette, mortar / homogenizer, ice and distilled water, EPT.

Product content:

extract: liquid 80 mL × 1 bottle, stored at 4 ° C. Reagent 1: Powder × 1 bottle, - 20 ° C protected from light. 2.4 mL of distilled water was added before, fully dissolved, and stored with income reagent recommended to save up - 20 ° C to avoid repeated freeze-thaw.
Reagents 2: Liquid 35 mL × 1 bottle, stored at 4 ° C.

Standard: Liquid 1 mL × 1, 5 μmol / ml of pair-nitrophenol, 4 ° C to prevent light.

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