Chinese Name	胶原酶Ⅱ
ALIAS	Paracetic peptide A
Appearance	Brown powder
Source	Prepared from cl.histolyticum
Solubility	5 mg/mlWater

 

 

Collagenase II is suitable for the separation of fat, liver, bone, thyroid, heart and salivary gland gland tissue cells.

collagenase is derived from the soluble tissue dehydramia

Collagen enzyme Ⅱ can be used by Tesca Buffer (50 mm TES, 0.36 mm calcium chloride, pH 7.4,,, pH 7.4,, PH 7.4,,, pH 7.4,,, PH 7.4, 37 ° C) Matching 1-2 mg/ml. You can also use calcium -magnesium -containing PBS, calcium magnesium Hanks, or serum -free training. After the matching is finished, avoid repeated freezing.
1 collagen digestive unit (CDU): under the conditions of pH 7.4, 37 ° C, and calcium ions, the polypeptide released from the collagen every 5 hours is equivalent to the amount of ketone coloring 1 microline. It is an active unit. 1FalgPa unit: Under the conditions of PH 7.5, 25 ° C and calcium ions, a Falgpa hydrolyzed unit can hydrolyze 1.0µmol acyrane-Leu-Gly-Pro-ALA.
Collase Ⅱ is a rough product, which is used to separate fat cells. It can also be used to separate various tissues and tumors, especially epithelial tissues. The external view is black brown powder. Molecular weight is 68,000 to 125,000, and the most suitable pH is 6.3-8.8. The collagen enzyme can be specific to bind the peptide bonds between the neutral amino acids (X) and glycine in the-R-PRO-8-X-GLY-PRO-R-sequence. This rough product is a mixed enzyme secreted by the soluble tissue. Mainly collagense, neutral protease and dehioxylinase. Among them, dermal proteases are oxidized and most lively enzymes. The separation of tissue must rely on collagense and neutral protease.

Activant: CA2+, each molvrase needs 4g of calcium ions to activate.

Inhibitors: EGTA, reducing glutathione, β-cymbal ethanol, sodium cymbal ethyl, 8-hydroxylinoline, 2,2 \u0026#39; -lianyridine, etc.

Different types: Different types of collagen are their natural substrates, and some synthetic polypeptides can also be used as their substrates, such as: N-CBZ-GLY-Pro-Gly-PRO-ALA (KM (KM (KM (KM (KM (KM (KM \u003d 0.71 mm), N-CBZ-GLY-PRO-Leu-Leu-GLY-PRO, N- (3- (2-Furyl) acryloyl)-leu-gly-pro-ala (FALGPA), 4-PHENYLAZO BENZYLORBORBORBONYL-PRO- Leu-GLY-PRO-D-RG, N-2,4-Dinitrophenyl-Pro-GLN-GLY-ALA-GLY-GLN-D-RG. In addition, N-Succinyl-Gly-Pro-Leu-GlyPro7-AMIDO-4-Methylcoumarin is a substrate for peptidases of collagenase.N- (2,4-Dinitropheneyl) -Proleu-Gly-Leu-TRP-ALA-ALA-ALA-ALG amino acid compound is a substrate for vertebrate colloidase.

\ Remarks: Product information may be optimized and upgraded.Please refer to the actual label information.

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