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Lysostaphin
Lysostaphin
溶葡球菌酶
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Chinese Name 溶葡球菌酶
Cas No. 9011-93-2
Titer 500U/mg

 

 

 

In 1964, Schindler and Schuhard were first discovered in the culture of STAPHYLOCCUS SIMULANS (US patent, patent number 3,278,378; 1966), with a molecular weight of 27 kda and containing 246 amino acids. Its mechanism is to lift the glycine five skin bonding bridges in the glycopylide polyaccharum, and then destroy the integrity of the cell wall and dissolved the bacteria, thereby achieving the purpose of quickly dissolving and killing bacteria. Its unique sterilization mechanism is not easy to induce and generate resistance to resistance to resist The strain is a peptide chain. The molecular weight of the natural soluble staphylococcus enzyme is 27 kda and contains 246 amino acids.

Enzyme activity measurement method: determine through the turbidity method
Cracking Staphylococcus aureus: 4 ~ 32U/ml
Cracking Epitrobacter Staphylococci: 8 ~ 64U/ML
Solubility: Soluble at 50mmol/L pH 7.5 Tris-HCL
solution storage method: PH7.5 enzyme solution (pH5 ~ 8) is placed at 4 ℃, basically stable within 2-3 days
Properties (the following information is for reference only) : White or pale yellow powder, a metal protease of zinc containing, is 9.55 for the electric point. Hydroxybenzoic acid (PCMB), benzozoosulfonal (PMSF), and sulfulse sulfuritol (DTT), etc., under the condition of 1 mmol/L, have no significant effect on enzyme vitality. ; BA ions, MN ions, CA ions, MG ions, and EDTA show a certain degree of inhibitory effect on the iconicococcus enzyme under 1 mmol/L.

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