Chinese Name

蛋白酶抑制剂混合物(植物样品抽提用, 100X)

 

 

 

Product description:

Plant cells or tissue extracts contain many endogenous proteases, phosphatase, etc. , Thus affecting subsequent protein detection. Therefore, adding proper proteases, phosphates and other inhibitors to the extract is an effective way to prevent protein degradation and decorative modification. This product is a protease inhibitor mixture extracted by plant cells or tissue protein, which contains broad -spectrum lingine, cysteine \u200b\u200band acidic protease inhibitors, and amino peptidase inhibitors. The mixture of protease inhibitors (plant samples, 100X) is used to the cracking solution at a ratio of 1: 100, which can be used for extraction of plant cells or tissue proteins and effectively inhibit protein degradation.

Scope of application:

Various protease activity in plant cells or tissue extracts, such as lingicate, amino peptide, cysteine \u200b\u200bammonia Portase, soure, and citrinine, metal protease, etc. Suitable for Western blot and immune co -sedimentation to detect phosphate protein, protein kinase activity measurement, etc.

How to use:

1. Mixture of protease inhibitors (plant sample extraction, 100X), according to the proportion of 1: 100 during use, respectively Add to the cracking solution and mix it. The cracking solution containing protease phosphatase inhibitors should be available for existing, and it should not be prepared for subsequent use after freezing.

2. Because the metal protease inhibitors in the product can form red or purple chelates with binary metal ions such as ferrous iron, the product may show light pink; if the cracks or samples It contains a higher concentration of metal ions such as railways, which may have darker red, but does not affect use.

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