Product Name	ATG9A
Chinese Name	自噬相关蛋白9ARecombinant rabbit monoclonal anti
Alias	ATG9A_HUMAN; Autophagy-related protein 9A; APG9-like 1; mATG9; APG9L1; MGD3208;
Research Area	Tumour  Cell biology  Signal transduction  TumourCell biologyMaker  Epigenetics
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	R4D8
React Species	(predicted: Human, )
Applications	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:50-100 ICC=1:50-100 IF=1:50-100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	94kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ATG9A
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Acts upstream of or within autophagosome assembly. Located in endosome; phagophore assembly site; and trans-Golgi network. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Cytoplasmic vesicle > autophagosome membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. SWISS: Q7Z3C6 Gene ID: 79065 Database links: Entrez Gene: 79065 Human SwissProt: Q7Z3C6 Human
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HepG2, 2: HeLa Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 94 kDa Observed MW: 100 kDa Tissue: Human thyroid cancer Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60696R

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