Product Name	RBX1
Chinese Name	Ring finger protein75Recombinant rabbit monoclonal anti
Alias	RBX1_HUMAN; E3 ubiquitin-protein ligase RBX1; EC:2.3.2.27; EC:2.3.2.32; RNF75; ROC1; E3 ubiquitin-protein transferase RBX1; Protein ZYP; RING finger protein 75; RING-box protein 1 (Rbx1); Regulator of cullins 1 (ROC1); E3 ubiquitin-protein ligase RBX1, N-terminally processed; E3 ubiquitin-protein transferase RBX1, N-terminally processed;
Research Area	Cell biology  Chromatin and nuclear signals  Epigenetics
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	R2D3
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-2000 IP=1:50-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:50-100 IF=1:50-100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	12kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RBX1
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010] Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Widely expressed. SWISS: P62877 Gene ID: 9978 Database links: Entrez Gene: 9978 Human Entrez Gene: 56438 Mouse Entrez Gene: 300084 Rat SwissProt: P62877 Human SwissProt: P62878 Mouse
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: HepG2, 3: HEK-293, 4: NIH/3T3, 5: PC-12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 12 kDa Observed MW: 12 kDa Tissue: Rat stomach Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60704R Tissue: Human bladder cancer Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60703R

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