Product Name	STIP1
Chinese Name	应激诱导磷蛋白1Recombinant rabbit monoclonal anti
Alias	Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-organizing protein; NY REN 11 antigen; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; STIP1_HUMAN; Stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; Transformation sensitive protein IEF SSP 3521; Transformation-sensitive protein IEF SSP 3521.
Research Area	Cell biology  immunology  Signal transduction
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	B5C7
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IP=1:50-100 IHC-P=1:100-500 Flow-Cyt=1:50-100 ICC=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	63kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human STIP1
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	PBS, Glycerol, BSA
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). SWISS: P31948 Gene ID: 10963
Product Picture	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Testis tissue lysates Lane 4: Human A549 cell lysates Lane 5: Human A431 cell lysates Lane 6: Human 293T cell lysates Lane 7: Human HepG2 cell lysates Lane 8: Human Jurkat cell lysates Primary: Anti-STIP1 (SLM-60759R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa Tissue: Human testis Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60759R Tissue: Rat testis Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60759R Cell line: Neuro-2a Fixation: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:500 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60759R Cell line: HeLa Fixation: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:500 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60759R Cell line: HeLa Fixation: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary Ab dilution: 1:100 Secondary Ab: Goat Anti-Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody(Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for

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