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Product Name Keratin 14 Immunogen Species Rabbit Clonality Monoclonal Clone NO. A5C9 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:1000-1:5000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 角蛋白14(Keratin 14, K14)是角蛋白家族中的一员,与角蛋白5组成一组角蛋白对。细胞角蛋白14在鳞状细胞源性Tumour中有表达。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature.
Lysate: Rat skin, Mouse skin
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 52 kDa
Observed MW: 52 kDa
Tissue: Human prostate
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60225R
Tissue: Human esophagus
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60225R
Tissue: Human breast
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60225R
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