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Product Name Keratin 7 Chinese Name Immunogen Species Rabbit Clonality Monoclonal Clone NO. B10D5 React Species (predicted: Human, ) Applications WB=1:500-1:2000 IHC-P=1:100-500 ICC=1:50-1:100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 角蛋白(细胞角蛋白)是主要在epithelial cells中表达的中间丝蛋白。角蛋白的异二聚体由一个酸性角蛋白(或 I 型角蛋白,角蛋白9 至 23)和碱性角蛋白(或 II 型角蛋白,角蛋白 1 至 8)组成聚合形成纤丝。角蛋白同工型的组织特异性和分化特异性表达,使其可用作研究生物标记物。研究证实,角蛋白基因中的突变与皮肤疾病、肝与胰疾病和炎性肠病相关。角蛋白 7 和角蛋白 19 存在于肝脏和胰腺祖细胞/Stem cells中。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Lysate: Hela
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 51 kDa
Observed MW: 51 kDa
Tissue: Human breast
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60218R
Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60218R
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