Product Name	Nuclear Matrix Protein p84
Chinese Name	核基质p84蛋白Recombinant rabbit monoclonal anti
Alias	Death domain containing protein p84N5; HPR 1; HPR1; hTREX84; Nuclear matrix protein p84; P84; P84N5; Tho 1; THO complex 1; THO complex subunit 1; Tho1; THOC 1; THOC1; THOC1_HUMAN.
Immunogen Species	Rabbit
Clonality	Monoclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-1000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	72kDa
Cellular localization	The nucleus cytoplasmic
Form	Lyophilized or Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Nuclear Matrix Protein p84
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This protein is part of the THO complex (THOC). The THO complex is recruited to transcribed genes and travels with the RNA polymerase during elongation. It may physically link proteins that function in transcription and in RNA export and RNA processing. Function: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. Subunit: Component of the THO complex, which is composed of THOC1, THOC2, THOC5, THOC6 and THOC7. Together with THOC3, ALYREF/THOC4 and DDX39B, THO forms the transcription/export (TREX) complex. Binds to the hypophosphorylated form of RB1. Interacts with THOC2, DDX39B and RNA polymerase II. Subcellular Location: Isoform 1: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution. Isoform 2: Cytoplasm. Tissue Specificity: Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. Post-translational modifications: Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. Similarity: Contains 1 death domain. SWISS: Q96FV9 Gene ID: 9984 Database links: Entrez Gene: 9984 Human Entrez Gene: 225160 Mouse Entrez Gene: 291797 Rat Omim: 606930 Human SwissProt: Q96FV9 Human SwissProt: Q8R3N6 Mouse SwissProt: P59924 Rat Unigene: 712543 Human Unigene: 219648 Mouse Unigene: 127881 Rat
Product Picture	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human U251 cell lysates Lane 4: Human HUVEC cell lysates Lane 5: Human LOVO cell lysates Primary: Anti-Nuclear Matrix Protein p84 (SLM-54202R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 76 kDa Sample: Lane 1: Mouse Placenta tissue lysates Lane 2: Rat Placenta tissue lysates Lane 3: Human HeLa cell lysates Lane 4: Human HepG2 cell lysates Primary: Anti-Nuclear Matrix Protein p84 (SLM-54202R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 75 kDa

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