Rabbit Anti-CCR7 antibody
CCR7_HUMAN; BLR 2; BLR2; C C chemokine receptor type 7; C C CKR 7; CC chemokine receptor 7; CC chemokine receptor type 7; CC CKR 7; CCCKR7; CCR 7; CD 197; CD197; CD197 antigen; CDW197; Chemokine C C motif receptor 7; Chemokine C C receptor 7; Chemokine re
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Product Name CCR7 Chinese Name 细胞表面Chemokine受体7Recombinant rabbit monoclonal anti Alias CCR7_HUMAN; BLR 2; BLR2; C C chemokine receptor type 7; C C CKR 7; CC chemokine receptor 7; CC chemokine receptor type 7; CC CKR 7; CCCKR7; CCR 7; CD 197; CD197; CD197 antigen; CDW197; Chemokine C C motif receptor 7; Chemokine C C receptor 7; Chemokine receptor 7-like protein; EBI 1; EBI1; Ebi1h; EBV Induced G Protein Coupled Receptor 1; Epstein Barr virus induced G protein coupled receptor; Epstein Barr virus induced gene 1; EVI 1; EVI1; Lymphocyte Specific G Protein Coupled Peptide Receptor; MGC108519; MIP 3 beta receptor; MIP3 Beta Receptor. Research Area immunology Signal transduction G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Monoclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:50-200 Flow-Cyt=1ug/Test ICC=1:100 IF=1:50-200 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CCR7 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. [provided by RefSeq, Jul 2008]
Function:
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P47774
Gene ID:
1236
Database links:Entrez Gene: 1236 Human
Entrez Gene: 12775 Mouse
Omim: 600242 Human
SwissProt: P32248 Human
SwissProt: P47774 Mouse
Unigene: 370036 Human
Unigene: 2932 Mouse
Unigene: 229736 Rat
Chemokine是当今cell factorResearch Area的热点之一,它参与多种免疫及炎症反应,在感染、Tumour的生长与转移、组织修复及创伤愈合等病理生理过程中发挥重要作用.CCR7在Tumour的生长与转移方面起到一定的作用.Product Picture 
Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Mouse) Lysate at 40 ug
Lane 4: Lung (Mouse) Lysate at 40 ug
Lane 5: Thymus (Rat) Lysate at 40 ug
Lane 6: Spleen (Rat) Lysate at 40 ug
Lane 7: Lymph node (Rat) Lysate at 40 ug
Lane 8: Lung (Rat) Lysate at 40 ug
Lane 9: Jurkat (Human) Cell Lysate at 30 ug
Lane 10: MCF-7 (Human) Cell Lysate at 30 ug
Lane 11: Hela (Human) Cell Lysate at 30 ug
Lane 12: HL60 (Human) Cell Lysate at 30 ug
Primary: Anti-CCR7 (SLM-52774R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Western blot analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (SLM-52774R, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate
All lanes: Western blot analysis of CCR7 with anti-CCR7 antibody at 1:5,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate (10 µg).
Lane 3/4: CCR7 fragment 1 knockdown Hela whole cell lysate (10 µg).
Lane 5/6: CCR7 fragment 2 knockdown Hela whole cell lysate (10 µg).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (SLM-52774R, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( SLM-52774R, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC staining of CCR7 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (SLM-52774R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of CCR7 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (SLM-52774R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Blank control（black line）:Jurkat.
Primary Antibody (green line): Mouse Anti-CCR7 antibody (SLM-52774R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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