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Product Name TGF alpha Chinese Name 转移生长因子α抗体 Alias EGF like TGF; ETGF; TFGA; TGF A; TGF type 1; TGFA; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1; TGFA_HUMAN; TGFα; TGF-α; TGF α. Research Area Tumour Cardiovascular Signal transduction Growth factors and hormones cell factor Immunogen Species Rabbit Clonality Monoclonal React Species (predicted: Human, ) Applications WB=1:500 IP=1:50 IHC-P=1:100-500 ICC=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 17kDa Cellular localization The cell membrane Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen Recombinant human TGF alpha Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Function:
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar (By similarity). Inhibitor of acid secretion. Inhibitor of aminopyrine uptake in parietal cells (in vitro).
Subunit:
Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2.
Subcellular Location:
Transforming growth factor alpha: Secreted, extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
Similarity:
Contains 1 EGF-like domain.
SWISS:
P01135
Gene ID:
7039
Database links:
Entrez Gene: 7039 Human
Entrez Gene: 21802 Mouse
Omim: 190170 Human
SwissProt: P01135 Human
SwissProt: P48030 Mouse
Unigene: 170009 Human
Unigene: 137222 Mouse
Unigene: 9952 Rat
Product Picture Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.ICC staining TGF alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC staining TGF alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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