Product Name	NCAM
Chinese Name	神经Cell adhesion molecule1Recombinant rabbit monoclonal anti
Alias	CD56 antigen; Cell Adhesion Molecule Neural 1; MSK39; N CAM 120; N CAM 140; N CAM 180; NCAM 1; NCAM 120; NCAM 140; NCAM1; Neural cell adhesion molecule 1 120 kDa isoform; Neural cell adhesion molecule 1 140 kDa isoform; Neural cell adhesion molecule 1; NCAM1_HUMAN.
literatures	Specific References  (1)     |     SLM-52824R has been referenced in 1 publications. [IF=9.429] Zhang Yufan. et al. Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22  WB ;  Mouse.   PubMed:36329527
Research Area	Cell biology  Neurobiology  Stem cells  Cell type markers
Immunogen Species	Rabbit
Clonality	Monoclonal
React Species	Human,  (predicted: Zebrafish, )
Applications	WB=1:500-2000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	92kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NCAM
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011] Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Subcellular Location : Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. Similarity: Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P13591 Gene ID: 4684 细胞粘附蛋白（Call Adhesion Protein） 神经元Maker NCAM-1为神经Cell adhesion molecule，主要分布于神经组织，神经—肌肉接头，神经—内分泌腺和某些内分泌腺以及大多数神经外胚层来源的细胞、组织和Tumour中。 NCAM 1主要用于视网膜母细胞瘤、髓母细胞瘤、星形细胞瘤、神经母细胞瘤等Tumour方面的研究。CD56（神经细胞黏附分子，NCAM）表达于大部分神经外胚层来源的细胞系、组织和Tumour如视网膜母细胞瘤、成神经管细胞瘤、星形细胞瘤以及成神经细胞瘤。它也表达于一些中胚层来源的Tumour如横纹肌肉瘤。NK细胞以及NK细胞淋巴瘤为阳性。CD56被认为是NK细胞Tumour的重要Maker。该抗体识别分子量为180，145和125kDa亚型。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: SH-SY5Y, 2: Mouse cerebellum, 3: Rat cerebellum Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 95 kDa Observed MW: 140-180 kDa Western blot analysis of NCAM/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM/CD56 antibody (SLM-52824R) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 95 kDa Observed band size: 120/140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Western blot analysis of NCAM/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody (SLM-52824R) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-NCAM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (SLM-52824R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NCAM ) monoclonal Antibody, Unconjugated (SLM-52824R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Cell line: SH-SY5Y Fixation: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary Ab dilution: 1:100 Secondary Ab: Goat Anti-Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for SLM-52824R Flow cytometric analysis of NCAM/CD56 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (SLM-52824R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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