Product Name	MYH6
Chinese Name	心肌肌球蛋白重链Recombinant rabbit monoclonal anti
Alias	MYH6_HUMAN; Myosin-6; MYHCA; Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC; Myosin heavy chain;
literatures	Specific References  (1)     |     SLM-52867R has been referenced in 1 publications. [IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858  WB ;  Mouse.   PubMed:37039469
Research Area	Cardiovascular  immunology
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	8C1
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IF=1:100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	224kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human MYH6
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Feb 2017] Subcellular Location: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. SWISS: P13533 Gene ID: 4624 Database links: Entrez Gene: 4624 Human Entrez Gene: 17888 Mouse Entrez Gene: 29556 Rat SwissProt: P13533 Human SwissProt: Q02566 Mouse SwissProt: P02563 Rat
Product Picture	Western blot analysis of Myosin heavy chain on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse smooth muscle tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Human striated muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (SLM-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

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