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Product Name EEF1A1 Chinese Name 翻译延伸因子EF-1aRecombinant rabbit monoclonal anti Alias Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu (EF-Tu); Eukaryotic elongation factor 1 A-1 (eEF1A-1); Leukocyte receptor cluster member 7; EF1A1_HUMAN; EEF1A; EF1A; LENG7; Immunogen Species Rabbit Clonality Monoclonal Clone NO. R8F9 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-2000 ICC=1:50-100 IF=1:50-100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 50kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human eEF1a1 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
SWISS:
P68104
Gene ID:
1915
Database links:
Entrez Gene: 1915 Human
Entrez Gene: 13627 Mouse
SwissProt: P68104 Human
SwissProt: P10126 Mouse
Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: MCF7, 2: PANC-1, 3: Neuro-2a, 4:
Rat kidney
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 50 kDa
Observed MW: 55 kDa
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