Product Name	[KO validated anti] TAGLN2
Chinese Name	Cytoskeleton相关蛋白2抗体
Alias	CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin 2; Transgelin-2.
Research Area	Tumour  Cell biology  TumourCell biologyMaker
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,
Applications	WB=1:500-2000 ICC=1:100 IF=1:50-200  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	cytoplasmic Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human TAGLN2: 1-199/199
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene. Similarity: Belongs to the calponin family. Contains 1 calponin-like repeat. Contains 1 CH (calponin-homology) domain. SWISS: P37802 Gene ID: 8407 Database links: Entrez Gene: 8407 Human Omim: 604634 Human SwissProt: P37802 Human Unigene: 517168 Human
Product Picture	Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug Lane 2: TAGLN2 knockout (KO) 293T (Human) Cell Lysate at 25 ug Primary: Anti-TAGLN2 (SL55211R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 18 kD Observed band size: 20 kD Sample: Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Placenta (Mouse) Lysate at 40 ug Lane 3: Uterus (Mouse) Lysate at 40 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Lane 6: U87MG (Human) Cell Lysate at 30 ug Lane 7: U2os (Human) Cell Lysate at 30 ug Lane 8: Huvec (Human) Cell Lysate at 30 ug Lane 9: A673 (Human) Cell Lysate at 30 ug Primary: Anti-TAGLN2 (SL55211R) at 1/1000 dilution Anti-beta-Actin (SL0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 22 kD MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)TAGLN2 polyclonal Antibody, Unconjugated (SL55211R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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