Product Name	[KO validated anti] SQSTM1/p62
Chinese Name	UbiquitinBinding proteinP62抗体
Alias	SQSTM_HUMAN; Sequestosome-1; ORCA; OSIL; EBI3-associated protein of 60 kDa (EBIAP; p60); Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62;
literatures	Specific References  (11)     |     SL55207R has been referenced in 11 publications. [IF=7.419] Wei-Wei Zhou. et al. Gentianella acuta improves TAC-induced cardiac remodelling by regulating the Notch and PI3K/Akt/FOXO1/3 pathways. BIOMED PHARMACOTHER. 2022 Oct;154:113564  WB ;  Rat.   PubMed:35988427 [IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078  WB ;  Mouse.   PubMed:35489469 [IF=5.396] Kaidi Ma. et al. Lactobacillus rhamnosus GG ameliorates deoxynivalenol-induced kidney oxidative damage and mitochondrial injury in weaned piglets. Food Funct. 2022 Mar;:  WB ;  Piglets.   PubMed:35285834 [IF=4.932] Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096  WB ;  Human.   PubMed:34464886 [IF=4.872] Jianzhao Liao. et al. Copper induces energy metabolic dysfunction and AMPK-mTOR pathway-mediated autophagy in kidney of broiler chickens. Ecotox Environ Safe. 2020 Dec;206:111366  WB ;  Chicken.   PubMed:33010598 [IF=3.739] Lu Peng. et al. Polychlorinated Biphenyl Quinone Metabolites Cause Neutrophil Extracellular Traps in Mouse Bone Marrow Neutrophils. Chem Res Toxicol. 2022;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:35168318 [IF=3.674] Qiao Wanchen. et al. Liensinine ameliorates ischemia–reperfusion-induced brain injury by inhibiting autophagy via PI3K/AKT signaling. FUNCT INTEGR GENOMIC. 2023 Jun;23(2):1-9  WB ;  Rat.   PubMed:37118322 [IF=3.115] Pengfei Zhang. et al. The Effects of Ginkgo biloba Extract on Autophagy in Human Macrophages Stimulated by Cigarette Smoke Extract. FRONT BIOSCI-LANDMRK. 2023 Mar;28(3):50  WB ;  Human.   PubMed:37005757 [IF=2.952] Zhi-Yu Li. et al. NOX4 stimulates ANF secretion via activation of the Sirt1/Nrf2/ATF3/4 axis in hypoxic beating rat atria. Mol Med Rep. 2022 Mar;25(3):1-9  WB ;  Rat.   PubMed:35029280 [IF=2.677] Xing Yifeng. et al. Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy. J ORTHOP SURG RES. 2023 Dec;18(1):1-13  WB ;  Rat.   PubMed:36814286 [IF=2.65] Ying Li. et al. Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively.. EVID-BASED COMPL ALT. 2022 Nov;2022:4263417-4263417  WB ;  Rat.   PubMed:36437825
Research Area	Cardiovascular  Chromatin and nuclear signals  Signal transduction  Apoptosis  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:50-100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38/47kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human SQSTM1/p62: 1-440
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009] Function: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Subunit: Cytoplasm. Late endosome. Nucleus. Endoplasmic reticulum. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum. Subcellular Location: Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. DISEASE: Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years. Similarity: Contains 1 OPR domain. Contains 1 UBA domain. Contains 1 ZZ-type zinc finger. SWISS: Q13501 Gene ID: 8878 Database links: Entrez Gene: 8878 Human Entrez Gene: 18412 Mouse Entrez Gene: 113894 Rat SwissProt: Q13501 Human SwissProt: Q64337 Mouse SwissProt: O08623 Rat
Product Picture	Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug Lane 2: SQSTM1 / p62 knockout (KO) 293T (Human) Cell Lysate at 25 ug Primary: Anti-SQSTM1 / p62 (SL55207R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 62 kD Observed band size: 62 kD Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Stomach (Mouse) Lysate at 40 ug Lane 3: Kidney (Mouse) Lysate at 40 ug Lane 4: Spleen (Mouse) Lysate at 40 ug Lane 5: Muscle (Mouse) Lysate at 40 ug Lane 6: Small intestine (Rat) Lysate at 40 ug Lane 7: Hela (Human) Cell Lysate at 30 ug Lane 8: A549 (Human) Cell Lysate at 30 ug Lane 9: Panc-1 (Human) Cell Lysate at 30 ug Primary: Anti-SQSTM1/p62 (SL55207R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (SL55207R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (SL55207R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human appendix); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (SL55207R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)SQSTM1/p62 polyclonal Antibody, Unconjugated (SL55207R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Totalgoods,subtotals:￥Checkout