Product Name	Phospho-HSD17B13 (Ser33)
Chinese Name	磷酸化17β羟基类固醇脱氢酶13抗体
Alias	HSD17B13(phospho S33); HSD17B13(phospho Ser33); phospho-HSD17B13(S33); p-HSD17B13(Ser33); 17-beta-HSD 13; 17-beta-hydroxysteroid dehydrogenase 13; DHB13_HUMAN; HMFN0376; Hsd17b13; SCDR9; Short-chain dehydrogenase/reductase 9; UNQ497/PRO1014.
Product Type	Phosphorylated anti
Research Area	Cell biology  Signal transduction  The new supersedes the old  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 Flow-Cyt=1ug/Test ICC=1:100  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	34kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human HSD17B13 around the phosphorylation site of Ser33
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	17 beta-HSD13 (17 beta hydroxysteroid dehydrogenase type 13), also designated Short-chain dehydrogenase/reductase 9 (SCDR9), belongs to the 17 beta-HSD family of proteins, which regulate the availability of steroids within various tissues throughout the body. 17 beta-HSD13 is a 300 amino acid secreted protein that is highly expressed in liver and is also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. The gene encoding 17 beta-HSD13 maps to chromosome 4, which houses nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Function: Highly expressed in the liver. Also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. Subcellular Location: Secreted. Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. SWISS: Q7Z5P4 Gene ID: 345275 Database links: Entrez Gene: 345275 Human Entrez Gene: 243168 Mouse Entrez Gene: 305150 Rat Omim: 612127 Human SwissProt: Q7Z5P4 Human SwissProt: Q8VCR2 Mouse SwissProt: Q5M875 Rat Unigene: 284414 Human Unigene: 284944 Mouse Unigene: 25104 Rat
Product Picture	Sample: Lane 1: Human U-2 OS cell lysates Lane 2: Human HL-60 cell lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Human U937 cell lysates Lane 5: Human HepG2 cell lysates Primary: Anti-Phospho-HSD17B13 (Ser33) (SL22308R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 35 kDa Blank control（black line）:HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-HSD17B13 (Ser33) antibody (SL22308R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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