Product Name	ATG5/APG5L
Chinese Name	自噬蛋白5单克隆抗体
Alias	ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN.
Research Area	Cell biology  Apoptosis  Autophagy
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	10C4
React Species	Human, Mouse, Rat,
Applications	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human ATG5 Protein
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis-specific protein or APS), function as substrates for the autophagy protein Apg12. These proteins are covalently bonded together to form Apg12/APG5 conjugates, which are required for the progression of autophagy. Function: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Subunit: The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate. Subcellular Location: Cytoplasm. Note=Colocalizes with nonmuscle actin. Tissue Specificity: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Post-translational modifications: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. Similarity: Belongs to the ATG5 family. SWISS: Q9H1Y0 Gene ID: 9474 Database links: Entrez Gene: 9474 Human Entrez Gene: 11793 Mouse Entrez Gene: 365601 Rat Omim: 604261 Human SwissProt: Q9H1Y0 Human SwissProt: Q99J83 Mouse SwissProt: Q3MQ06 Rat Unigene: 486063 Human Unigene: 22264 Mouse Unigene: 98385 Rat
Product Picture	Sample: Lane 1: Human PANC-1 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-ATG5/APG5L (SLM-33386M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 47 kDa Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (SLM-33386M) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-mouse IgG antibody (SL0296G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (SLM-33386M) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-mouse IgG antibody (SL0296G-AF488) for 90 minutes, and DAPI for nuclei staining.

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