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Product Name [KO validated anti] RHOC Chinese Name RhoC抗体 Alias ARH 9; ARH9; ARHC; H9; MGC1448; MGC61427; Oncogene RHO H9; Ras homolog gene family member C; RAS related homolog 9; RHO C; Rho cDNA clone 9; Rho related GTP binding protein RhoC; Rho-related GTP-binding protein RhoC; rhoC; RhoC GTPase; RHOC_HUMAN; RHOH 9; RHOH9; Small GTP binding protein RhoC. Research Area Tumour Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:450-100 IF=1:50-100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant human RHOC: 1-193/193 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Function:
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
Subunit:
Interacts with RTKN. Interacts with AKAP13, DIAPH1, PKN2, ROCK1 and ROCK2. Interacts with ARHGDIA.
Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cleavage furrow. Note=Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.
Similarity:
Belongs to the small GTPase superfamily. Rho family.
SWISS:
P08134
Gene ID:
389
Database links:Entrez Gene: 389 Human
Omim: 165380 Human
SwissProt: P08134 Human
Unigene: 502659 Human
Unigene: 658289 Human
Product Picture Sample:
Lane 1: 293T (Human) Cell Lysate at 25 ug
Lane 2: RHOC knockout (KO) 293T (Human) Cell Lysate at 25 ug
Primary: Anti-RHOC (SL55187R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: A431 (Human) Cell Lysate at 30 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Primary:
Anti- RHOC (SL55187R) at 1/1000 dilution
Anti-beta-Actin (SL0061R) at 1/2000 dilution
Secondary:
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)RHOC polyclonal Antibody, Unconjugated (SL55187R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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