Product Name	CD68
Chinese Name	CD68抗体
Alias	CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68 (microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP
Research Area	immunology  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, )
Applications	ELISA=1:5000-10000 Flow-Cyt=1ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Detection molecular weight	110-140 kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant mouse CD68 protein: 119-298/326
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008] SWISS: P31996 Gene ID: 12514 Database links: Entrez Gene: 968 Human Entrez Gene: 12514 Mouse Omim: 153634 Human SwissProt: P34810 Human SwissProt: P31996 Mouse Unigene: 647419 Human Unigene: 15819 Mouse
Product Picture	Blank control(black line):Raw264.7 Primary Antibody (green line): Rabbit Anti-CD68 antibody (SL42013R) Dilution: 1ug/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line)：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control(black line):Raw264.7 Primary Antibody (green line): Rabbit Anti-CD68 antibody (SL42013R) Dilution: 1ug/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line)：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:Raw264.7. Primary Antibody (green line): Mouse Anti-CD68 antibody (SL42013R) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:Raw264.7. Primary Antibody (green line): Mouse Anti-CD68 antibody (SL42013R) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag (Cat. SL42013P) at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Mouse CD68 Antibody, the EC50 is 142.5 ng/mL. Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag (Cat. SL42013P) at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Mouse CD68 Antibody, the EC50 is 175.4 ng/mL.

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