Product Name	Apoptosis Research Antibody Assembly kit
Chinese Name	凋亡研究抗体套装
Alias	Apoptosis Research Antibody Assembly kit(Bax,Caspase-3,Caspase-8,Caspase-9,Cytochrome C)  凋亡研究抗体套装,凋亡anti combination装
Research Area	Cell biology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Apoptosis is the most common type of programmed cell death. The signal transduction of apoptosis involves the processing and activation of cysteinase caspase and the alternative mitochondrial pathway mediated by pro-apoptosis factors. Among them, the caspase mediating the initiation of apoptosis includes caspase-8,-9,-10 and -12, in which caspase-8 is mainly involved in tumor necrosis factor receptor (TNFR) superfamily death receptor mediated extrinsic apoptosis pathway. Caspase-3,-6 and -7 mediate downstream effects, including cleavage of cytoskeleton and chromatin, and eventually lead to apoptosis. In addition, mitochondria also play key roles in activation of apoptosis. Different upstream stimuli promote the binding of pro-apoptosis factors to the outer membrane of mitochondria through signal transduction, which leads to the change of mitochondrial permeability and the release of inner mitochondrial membrane (IMM) proteins (such as cytochrome C and other proteins). Cytochrome C coupling activation of caspase-9 can further activate key downstream effector caspases. Apoptosis Research Antibody Assembly Kit is one economical package of selected antibodies for most representative apoptosis signaling pathway-related proteins, including caspase-8, Bax, cytochrome C, caspase-9 and caspase-3. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. Apoptosis Research Antibody Assembly kit Mouse Anti-Bax antibody (SLM-33279M) Rabbit Anti-Caspase 3 precursor antibody (SL2593R) Rabbit Anti-Caspase-8 subunit p10 antibody (SL20613R) Rabbit Anti-Caspase-9 antibody (SL20773R) Rabbit Anti-Cytochrome C antibody (SLM-52050R) SWISS: N/A Gene ID: N/A Database links: Apoptosis Research Antibody Assembly kit Mouse Anti-Bax antibody (SLM-33279M) Rabbit Anti-Caspase 3 precursor antibody (SL2593R) Rabbit Anti-Caspase-8 subunit p10 antibody (SL20613R) Rabbit Anti-Caspase-9 antibody (SL20773R) Rabbit Anti-Cytochrome C antibody (SLM-52050R) Apoptosis是一种最常见的细胞程序性死亡。凋亡的信号传导过程涉及半胱氨酸蛋白酶caspase 的加工和激活过程以及由促凋亡因子介导的Mitochondrion旁路途径。其中，介导凋亡起始的caspase包括caspase-8、-9、-10 和 -12，其中caspase-8主要参与由Tumour坏死因子受体（TNFR）超家族死亡受体介导的外源性凋亡途径。而介导下游效应的 caspase 包括 caspase-3、-6 和 -7，这些下游效应caspase 会导致Cytoskeleton蛋白和染色质被剪切并最终发生Apoptosis。另外，凋亡还可以通过Mitochondrion旁路途径发生，一些不同的上游应激通过信号传导过程促进促凋亡因子结合于Mitochondrion外膜，导致Mitochondrion通透性改变并释放Mitochondrion内膜蛋白（如细胞色素C等蛋白）。细胞色素C偶联激活的 caspase-9，会进一步激活下游关键效应的 caspase并最终发生Apoptosis。 凋亡研究抗体套装极具性价比，包含了最具代表性的凋亡信号通路相关蛋白，包括介导外源性凋亡途径的caspase-8、介导Mitochondrion旁路途径的促凋亡因子Bax和Mitochondrion内膜蛋白细胞色素C、参与多条凋亡分支途径的caspase-9以及介导下游效应的caspase-3。这些抗体在多种应用和物种中进行了测试。有关抗体具体使用信息，请参阅各个抗体产品数据表。
Product Picture	Primary: Anti-Caspase-9 (SL20773R) at 1/300 dilution Sample: K562 Cell (Human) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35/50 kD Observed band size: 35 kD Primary: Anti-Caspase 3 precursor  (SL2593R) at 1/1000 dilution Sample: Spleen (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Primary: Anti-Bax (SLM-33279M) at 1/1000 dilution Sample: 293T(Human) Cell Lysate at 30 ug 293T-UV(Human) Cell Lysate at 30 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD Primary: Anti- Caspase 8 (SL20613R) at 1/300 dilution Sample: Thymus (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12,55 kD Observed band size: 17 kD Primary: Anti- Cytochrome C (SLM-52050R) at 1/1000 dilution Sample: Liver (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/26 kD Observed band size: 12.8 kD Primary: Anti- Caspase 8 (SL20613R) Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Polyclonal Antibody, Unconjugated (SL20613R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. primary: Anti-Caspase-9 (SL20773R) Paraformaldehyde-fixed, paraffin embedded (Rat urinary bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (SL20773R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Primary: Anti- Cytochrome C (SLM-52050R) Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Polyclonal Antibody, Unconjugated (SLM-52050R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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