Product Name	EMT Research Antibody Assembly kit
Chinese Name	EMTMaker抗体套装
Alias	EMT Research Antibody Assembly kit(E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1)  上皮-间质转换(EMT)Maker抗体套装(E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1); EMTMaker抗体套装
Research Area	Tumour  Cell biology  Cell adhesion molecule  Cell Surface Molecule  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, )
Applications	IHC-P=1:100-500 IHC-F=1:400-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	For research use only. Not intended for diagnostic or therapeutic use.
PubMed	PubMed
Product Detail	Epithelial-mesenchymal transition (EMT) is a biological process that allows epithelial cell to assume mesenchymal phenotype. EMT and its reverse process, mesenchymal-epithelial transition (MET), are originally defined in the context of developmental stages and re-engaged in adults during wound healing, tissue regeneration, organ fibrosis, and cancer progression. Epithelial cells normally interacts with basement membrane via its basal surface. During EMT, they lose their cell polarity and cell-cell adhesion, and gain mesenchymal cell phenotype, including enhanced migratory capacity, invasiveness, elevated resistance to apoptosis, stem-like features, and increased production of ECM components. Accordingly, there is reprogrammed gene expression with EMT. Especially, some cytoskeleton proteins (such as cadherin family members), tight-junction proteins (such as Occludin and ZO-1) and intermediate filament of mesenchymal origin (such as Vimentin) undergo significant changes and serve as best EMT markers to evaluate EMT. EMT Research Antibody Assembly kit is one economical package of selected antibodies for most representative EMT markers, including E-Cadherin, N-Cadherin, Vimentin, Occludin, and ZO-1. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. EMT Research Antibody Assembly kit Rabbit Anti-E cadherin antibody (SL1519R) Rabbit Anti-N-cadherin antibody (SL1172R) Rabbit Anti-Vimentin antibody (SL0756R) Rabbit Anti-Occludin antibody (SL10011R) Rabbit Anti-ZO-1 antibody (SL1329R) SWISS: N/A Gene ID: N/A Database links: EMT Research Antibody Assembly kit Rabbit Anti-E cadherin antibody (SL1519R) Rabbit Anti-N-cadherin antibody (SL1172R) Rabbit Anti-Vimentin antibody (SL0756R) Rabbit Anti-Occludin antibody (SL10011R) Rabbit Anti-ZO-1 antibody (SL1329R) 上皮-间质转换(EMT)是一个使epithelial cells呈现间质细胞表型的生物学转变过程，EMT及其反向过程，间质-上皮转换(MET)，最初定义在早期发育阶段，并在成年期重新参与到伤口愈合、组织再生、器官纤维化和癌症进展等多种病理过程中。epithelial cells通常与基底膜紧密结合，在EMT过程中，它们失去细胞极性和细胞粘附并获得间质细胞表型，包括迁移能力、侵袭性、对凋亡的抗性增强、Stem cells样特征和ECM组分的增加。与此相应很多基因表达也发生了重新编程，尤其是一些Cytoskeleton蛋白(如钙粘素家族成员)、紧密连接蛋白(如Occludin和ZO-1)和间质特有中间纤维(如Vimentin)发生了显著变化。因此，这些蛋白可作为评价EMT的最佳标志。 EMTMaker抗体套装极具性价比，包含了最具代表性的EMT标记物，包括E-cadherin、N-cadherin、Vimentin、Occludin和ZO-1。这些抗体在多种应用和物种中进行了测试。有关抗体具体使用信息，请参阅各个抗体产品数据表。
Product Picture	Primary: Anti-Occludin (SL10011R) Paraformaldehyde-fixed, paraffin embedded (Normal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated (SL10011R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Primary: Anti- Vimentin (SL0756R) Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (SL0756R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Primary: Anti- E cadherin (SL1519R) Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL1519R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Primary: Anti- E cadherin (SL1519R) Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL1519R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Primary: Anti- ZO-1 (SL1329R) Independently Validated Antibody, image provided by Science Direct, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (SL1329R) at 1:250 followed by conjugation to the secondary antibody. Primary: Anti-N-cadherin (SL1172R) Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated(SL1172R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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