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Product Name Tumor Immunity Characterization Antibody Assembly kit Chinese Name Tumour免疫表征分析抗体套装 Alias Tumor immunity Characterization Antibody Assembly kit(CD4,CD8,PD-1,PD-L1,CTLA4) Tumour免疫表征分析抗体套装(CD4,CD8,PD-1,PD-L1,CTLA4) Research Area Tumour Cell biology immunology t-lymphocyte TumourCell biologyMaker Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Form Liquid Concentration 1mg/ml Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Recent clinical advances and basic discoveries have triggered a revolution in cancer immunotherapy. As a great breakthrough, some key immune checkpoints on the surface of tumor or immune cells were shown to play important roles in tumor immunity regulation and intervention of these targets will be beneficial to tumor therapy. Characterization of tumor infiltrating immune cells and tumor cells by examining immune cell phenotyping markers and immuno-checkpoints is important to efficacy of cancer immunotherapy. Among them, canonical helper T cell marker CD4, cytotoxic T cell marker CD8, PD-1 receptor on the surface of T cells, PD-1 ligand PD-L1 and CTLA-4 on the surface of tumor cells are top targets to be evaluated in tumor immunity research by different applications such as immuno-histochemistry (IHC) or Flow Cytometry (FCM).
Tumor Immunity Characterization Antibody Assembly kit is one economical package of selected antibodies against CD4, CD8,PD-1, PD-L1 and CTLA4 respectively. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet.
Tumor Immunity Characterization Antibody Assembly kit
Rabbit Anti-CD4 antibody (SL0647R)
Rabbit Anti-CD8 antibody (SL0648R)
Rabbit Anti-PD-1 antibody (SL1867R)
Rabbit Anti-PD-L1 antibody (SL4941R)
Rabbit Anti-CTLA4 antibody (SL10006R)
SWISS:
N/A
Gene ID:
N/A
Database links:Tumor Immunity Characterization Antibody Assembly kit
Rabbit Anti-CD4 antibody (SL0647R)
Rabbit Anti-CD8 antibody (SL0648R)
Rabbit Anti-PD-1 antibody (SL1867R)
Rabbit Anti-PD-L1 antibody (SL4941R)
Rabbit Anti-CTLA4 antibody (SL10006R)
基础研究的不断进步和最近的临床进展引发了一场癌症免疫治疗的革命。作为一项重大突破,Tumour或免疫细胞表面的一些关键免疫检查点分子在Tumour免疫调节中发挥着重要作用,对这些靶点进行干预将有利于Tumour的治疗。通过检测免疫细胞表面标志和多种免疫检查点的表达情况对Tumour浸润免疫细胞和Tumour细胞表征进行分析,对Tumour免疫治疗的疗效有重要意义。其中,经典的辅助性T细胞标志CD4、细胞毒性T细胞标志CD8、T细胞表面表达的PD-1受体、Tumour细胞表面表达的PD-L1(PD-1配体)和CTLA-4是在Tumour免疫研究中利用免疫组织化学(IHC)或流式细胞术(FCM)进行分析的重要指标。Tumour免疫表征分析抗体套装极具性价比,包含了分别用于检测CD4、CD8、PD-1、PD-L1和CTLA 4的抗体。这些抗体在多种应用和物种中进行了测试。有关抗体具体使用信息,请参阅各个抗体产品数据表。Product Picture Primary: Anti- CD4(SL0647R)
Sample:
HL60 Cell Lysate at 40 ug
Primary: Anti- CD4(SL0647R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 55kD
Primary: Anti-PD-L1 (SL4941R)
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-PD-L1(SL4941R)at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
Primary: Anti-PD-1 (SL1867R)
Sample:
Raji(Human) Cell Lysate at 30 ug
Thymus(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 40 ug
Thymus (Rat) Lysate at 40 ug
Primary: Anti-PD-1 (SL1867R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 58 kD
Primary: Anti-CD8(SL0648R)
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- CD8(SL0648R)at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 36 kD
Primary: Anti-CTLA4(SL10006R)
Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CTLA4 (SL10006R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-CD4 antibody (SL0647R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,dilution: 1μg /test.
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