Product Name	human CD15 (SSEA-1)
Chinese Name	小鼠抗人CD15单克隆抗体
Alias	Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	15F2
React Species	Human,
Applications	IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Lsotype	Mouse Ig1, k
Purification	Affinity purified by Protein G
Buffer Solution	0.01M TBS(pH=7.4)
Storage	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The CD15 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation. SWISS: / Gene ID: / Database links:
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (SLM-30016M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (SLM-30016M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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