Product Name	HSPB6
Chinese Name	热休克蛋白-20抗体
Alias	HSPB6_HUMAN; Heat shock protein beta-6; HSP20; Heat shock 20 kDa-like protein p20; heat shock protein family B (small) member 6; HEL55; Hsp20; PPP1R91;
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	A5H2
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-1:1000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	The nucleus The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG1/Kappa
Purification	Affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to nuclear foci during heat shock. SWISS: O14558 Gene ID: 126393 小的热休克蛋白作为分子伴侣，可能会使变性蛋白维持在折叠的能力状态，可能在各种生物过程中有多种功能。起到调节肌肉功能的作用，如平滑肌血管舒张和心肌细胞收缩。也可能调节与KDR相关的心肌血管生成。过度表达介导诱导损伤后的心脏保护和血管生成。稳定单体YWHAZ，从而支持YWHAZ类伴侣的活性。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition:4°C overnight Lysate:1: Mouse muscle, 2: Rat heart, 3: C2C12 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 17 kDa Observed MW: 17 kDa Tissue: Rat heart Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60365M Tissue: Mouse colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60365M Tissue: Human skeletal muscle Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60365M

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