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Product Name ECHS1 Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. H9F6 React Species (predicted: Human, ) Applications WB=1:500-1:1000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG1/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 该基因编码的蛋白质在Mitochondrion脂肪酸β氧化途径的第二步起作用。催化2-反式烯醇基辅酶A(CoA)中间体水合为L-3-羟酰基-CoA。该基因产物是水合酶/异构酶超家族的成员。定位于Mitochondrion基质。文献中已经描述了利用替代转录起始位点的转录变体。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition:4°C overnight
Lysate: HeLa, HepG2, 293, HT-29
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 31 kDa
Observed MW: 31 kDa
Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60362M
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60362M
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