Product Name	Annexin IV
Chinese Name	膜粘连蛋白A4重组鼠单抗
Alias	35 beta calcimedin; Carbohydrate binding protein P33/P41; DKFZp686H02120; Endonexin I; Lipocortin IV; P32.5; Annexin A4; Annexin IV placental anticoagulant protein II; ANX 4; ANX A4; ANX4; ANXA 4; LipocortinIV; P33/41; Placental anticoagulant protein II antibody PP4X; Proliferation inducing protein 28; ANXA4_HUMAN.
Research Area	Cardiovascular
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	D4C7
React Species	Human,
Applications	WB=1:200-1000 IHC-P=1:50-100 ICC=1:50 IF=1:50 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Annexin IV
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin A4 (ANXA4) belongs to the annexin family of calcium dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane related events along exocytotic and endocytotic pathways. ANXA4 has 45 to 59% identity with other members of its family and shares a similar size and exon intron organization. Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells. Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P09525 Gene ID: 307 Database links: Entrez Gene: 281625 Cow Entrez Gene: 606756 Dog Entrez Gene: 307 Human Entrez Gene: 11746 Mouse Entrez Gene: 79124 Rat Omim: 106491 Human SwissProt: P13214 Cow SwissProt: P50994 Dog SwissProt: P09525 Human SwissProt: P97429 Mouse SwissProt: P08132 Pig SwissProt: P55260 Rat Unigene: 422986 Human Unigene: 259702 Mouse Unigene: 19270 Rat
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition:4°C overnight Lysate: HeLa, HepG2, SW480, SH-SY5Y Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 32 kDa Observed MW: 32 kDa Tissue: Human placenta Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60359M Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60359M

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