Product Name	EHMT2
Chinese Name
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H4F2
React Species	(predicted: Human, )
Applications	WB=1:1000 ICC=1:50  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG2a/Kappa
Purification	Affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	组蛋白甲基转移酶，在常染色质中特异性地使组蛋白H3（分别为H3K9me1和H3K9me2）的“Lys-9”单甲基化和二甲基化。H3K9me代表了一个通过招募HP1蛋白到甲基化组蛋白的表观遗传转录抑制的特异性标签。还介导组蛋白H3（H3K56me1）的Lys-56在G1期的单甲基化，从而促进组蛋白H3和PCNA之间的相互作用并调节DNA复制。DNA甲基化也需要组蛋白甲基转移酶活性，DNA甲基化不需要组蛋白甲基转移酶活性，这表明这两种活性是独立发挥作用的。这可能通过不同的DNABinding protein如E2F6、MGA、MAX和/或DP1靶向组蛋白H3，也可能使组蛋白H1甲基化。除了组蛋白3753的非甲基化外，组蛋白3753的甲基化也介导组蛋白的甲基化。甲基化CDYL、WIZ、ACIN1、DNMT1、HDAC1、ERCC6、KLF12和自身。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Lysate: HeLa nucleus, 293T nucleus Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 150 kDa Observed MW: 150 kDa Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60315M

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