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Product Name PPP3CB Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. F4G2 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:1000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG1/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 丝氨酸/苏氨酸蛋白磷酸酶2B催化亚基亚型(PP2BB)是一种在人体内由PPP3CB基因编码的酶。这个亚基可能在钙调蛋白的激活中起作用。它是一种钙调素刺激的蛋白磷酸酶,在细胞内Ca2+信号的转导中起重要作用。它能去磷酸化激活转录因子NFATC1,去磷酸化激活转录因子ELK1。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: Mouse brain, 2: Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 59 kDa
Observed MW: 59 kDa
Tissue: Human brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60262M
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