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Product Name Calbindin D28K Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. B6G3 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus The cell membrane Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml Lsotype IgG1/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 可能刺激The cell membraneCa2+-ATP酶和3',5'-环核苷酸磷酸二酯酶。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Lysate: Mouse brain, Rat brain
Protein loading quantity: 20 μg
Exposure time: 15 s
Predicted MW: 28 kDa
Observed MW: 25 kDa
Tissue: Human cerebellum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60252M
Tissue: Rat cerebellum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60252M
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