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Product Name GCN5 Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. H8D2 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:1000 ICC=1:50-1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG2a/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 赖氨酸酰基转移酶,可作为乙酰转移酶、戊二酰转移酶或琥珀酸转移酶。作为组蛋白赖氨酸琥珀酸转移酶:催化组蛋白H3在“Lys-79”(H3K79succ)上的琥珀酰化,在基因的转录起始位点附近具有最大频率。组蛋白的琥珀酰化为表观遗传转录激活提供了一个特殊的标记。组蛋白琥珀酰化需要与提供琥珀酰辅酶A的2-酮戊二酸脱氢酶复合物结合。在不同的复合物中,既可以作为乙酰转移酶(HAT)也可以作为琥珀酰转移酶:在SAGA和ATAC复合物中,充当组蛋白乙酰转移酶。对核心组蛋白有明显的组蛋白乙酰转移酶活性,但与核小体核心颗粒不存在这种活性。组蛋白的乙酰化为表观遗传转录激活提供了一个特殊的标记。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: HepG2, 3: Neuro-2a, 4: EL4.IL-2, 5: BRL
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 95 kDa
Observed MW: 100 kDa
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