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Product Name Keratin 17 Chinese Name Immunogen Species Rabbit Clonality Monoclonal Clone NO. F2C10 React Species (predicted: Human, ) Applications WB=1:1000-1:5000 IHC-P=1:100-500 ICC=1:50-1:100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail I型角蛋白参与各种皮肤附件的形成和维持,特别是确定头发的形状和方向。通过与衔接子蛋白SFN结合并刺激Akt / mTOR途径来调节蛋白质的合成和epithelial cells的生长。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa, A431
Protein loading quantity: 20 μg
Exposure time: 15 s
Predicted MW: 48 kDa
Observed MW: 48 kDa
Tissue: Human prostate hyperplasia
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60232R
Tissue: Human cervical carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60232R
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