Product Name	ROCK2
Chinese Name	Rho相关蛋白激酶2单克隆抗体
Alias	p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; coiled-coil-containing protein kinase 2; KIAA0619; Rho associated, coiled coil containing protein kinase II; Rho-associated; Rho-associated protein kinase 2; Rock2; ROCK2_HUMAN.
Research Area	Tumour  Cell biology  Signal transduction  Apoptosis  Kinases and Phosphatases
Immunogen Species	Mouse
Clonality	Monoclonal
React Species	Human,  (predicted: Mouse, )
Applications	WB=1:500-1000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	161kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ROCK2
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]. Function: Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction. Subcellular Location: Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation at Tyr-722 reduces its binding to RHOA and iscrucial for focal adhesion dynamics. Dephosphorylation by PTPN11stimulates its RHOA binding activity. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thrprotein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. SWISS: O75116 Gene ID: 9475 Database links: Entrez Gene: 9475 Human Entrez Gene: 19878 Mouse Entrez Gene: 25537 Rat Omim: 604002 Human SwissProt: O75116 Human SwissProt: P70336 Mouse ROCK2（Rho kinase alpha）是ROCK1的同工酶。是一个丝/苏氨基酸激酶，可调节cell factor，平滑肌收缩和肌动蛋白的形成。
Product Picture	Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human Hela cell lysates Primary: Anti-ROCK2 (SLM-60013M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 161 kDa Observed band size: 180 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 4°C overnight Lysate: 1: HeLa, 2: MCF-7, 3: NIH/3T3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 161 kDa Observed MW: 161 kDa

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