TEL: +86 571 56623320 EMAIL: [email protected]
Product Name CD27 Chinese Name CD27Recombinant rabbit monoclonal anti Alias CD 27; CD27 antigen; CD27 molecule; CD27_HUMAN; CD27L receptor; LPFS2; MGC20393; OTTHUMP00000238557; S152; T cell activation antigen CD27; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF 7; TNFRSF7; TNFSF7; Tp 55; Tp55; Tumor necrosis factor receptor superfamily member 7. literatures Specific References (1) | SLM-54318R has been referenced in 1 publications.[IF=5.029] Hanhan Fang. et al. Gut-Spleen Axis: Microbiota via Vascular and Immune Pathways Improve Busulfan-Induced Spleen Disruption | mSphere. MSPHERE. 2022 Dec;: IF ; Mouse.Research Area Tumour immunology Stem cells lymphocyte t-lymphocyte cell factor Immunogen Species Rabbit Clonality Monoclonal Clone NO. 3C3 React Species Mouse, (predicted: Human, Rat, ) Applications WB=1:500-1000 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=1:50-100 ICC=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CD27 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
Function:
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Subunit:
Homodimer. Interacts with SIVA1 and TRAF2.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Found in most T-lymphocytes.
Post-translational modifications:
Phosphorylated.
O-glycosylated with core 1 or possibly core 8 glycans.
Similarity:
Contains 3 TNFR-Cys repeats.
SWISS:
P26842
Gene ID:
939
Database links:Entrez Gene: 939 Human
Entrez Gene: 21940 Mouse
Omim: 186711 Human
SwissProt: P26842 Human
SwissProt: P41272 Mouse
Unigene: 355307 Human
Unigene: 367714 Mouse
Unigene: 134200 Rat
Product Picture Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Thymus (Mouse) Lysate at 40 ug
Lane 4: Plasma (Mouse) at 40 ug
Primary: Anti- CD27 (SLM-54318R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD27) Monoclonal Antibody, Unconjugated (SLM-54318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD27) Monoclonal Antibody, Unconjugated (SLM-54318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode