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Product Name Mad2L1 Chinese Name Mad2L1Recombinant rabbit monoclonal anti Alias HSMAD2; Human mitotic feedback control protein Madp2 homolog mRNA complete cds; MAD 2; MAD2 like 1; MAD2 mitotic arrest deficient like 1; MAD2B; MAD2L1; Mitotic arrest deficient 2; Mitotic spindle assembly checkpoint protein MAD2A; REV7; MD2L1_HUMAN. 有丝分裂阻滞缺陷2样蛋白1重组兔单克隆抗体 Research Area Tumour Cell biology immunology Cyclin Immunogen Species Rabbit Clonality Monoclonal Clone NO. 6C6 React Species Human, Applications WB=1:500-2000 ICC=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 23kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen Recombinant human Mad2L1 protein Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]
Function:
Component of the spindle-assembly checkpoint thatprevents the onset of anaphase until all chromosomes are properlyaligned at the metaphase plate. Required for the execution of themitotic checkpoint which monitors the process ofkinetochore-spindle attachment and inhibits the activity of theanaphase promoting complex by sequestering CDC20 until allchromosomes are aligned at the metaphase plate.
Subunit:
Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. Binds to UBD during mitosis. Interacts with isoform 1 and isoform 2 of NEK2.
Subcellular Location:
Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.
Post-translational modifications:
Phosphorylated on multiple serine residues. The level ofphosphorylation varies during the cell cycle and is highest duringmitosis. Phosphorylation abolishes interaction with MAD1L1 andreduces interaction with CDC20. Phosphorylated by NEK2.
Similarity:
Belongs to the MAD2 family. Contains 1 HORMA domain.
SWISS:
Q13257
Gene ID:
4085
Database links:Entrez Gene: 4085 Human
Omim: 601467 Human
SwissProt: Q13257 Human
Unigene: 591697 Human
Product Picture Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution.
Positive control:
Lane 1: K562
Lane 2: 293T
Lane 3: SH-SY5Y
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Mad2L1) monoclonal Antibody, Unconjugated (SLM-54219R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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