Product Name	Vinculin (Loading Control)
Chinese Name	粘着斑蛋白（内参）Recombinant rabbit monoclonal anti
Alias	CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; OTTHUMP00000019861; OTTHUMP00000019862; VCL; VINC; VINC_HUMAN。
Product Type	Internal reference anti Recombinant rabbit monoclonal anti
Research Area	Cardiovascular  Cell biology  Signal transduction  Cell adhesion molecule  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	5G7
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IP=1:10-50 ICC=1:50 IF=1:50-200  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	124kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant Human Vinculin
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] Function: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Subunit: Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Tissue Specificity: Metavinculin is muscle-specific. Post-translational modifications: Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity). Aceylated; mainly by myristic acid but also small amount of palmitic acid (By similarity). DISEASE: Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Similarity: Belongs to the vinculin/alpha-catenin family. SWISS: P18206 Gene ID: 7414 Database links: Entrez Gene: 7414 Human Entrez Gene: 22330 Mouse Entrez Gene: 305679 Rat Omim: 193065 Human SwissProt: P18206 Human SwissProt: Q64727 Mouse SwissProt: P85972 Rat Unigene: 643896 Human Unigene: 279361 Mouse Unigene: 164613 Rat
Product Picture	Sample: Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug Lane 2: Skeletal muscle (Rat) Lysate at 40 ug Lane 3: Bladder (Mouse) Lysate at 40 ug Lane 4: Bladder (Rat) Lysate at 40 ug Lane 5: U251 (Human) Cell Lysate at 30 ug Lane 6: HUVEC (Human) Cell Lysate at 30 ug Lane 7: A431 (Human) Cell Lysate at 30 ug Lane 8: HepG2 (Human) Cell Lysate at 30 ug Lane 9: Spleen (Mouse) Lysate at 40 ug Lane 10: Spleen (Rat) Lysate at 40 ug Lane 11: Kidney (Mouse) Lysate at 40 ug Lane 12: A549 (Human) Cell Lysate at 30 ug Primary: Anti-Vinculin (SLM-54148R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD

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